What is the CytoTune™-iPS Reprogramming Kit?

By continuing to browse the site, you are agreeing to our use of cookies.Supporting Products and References for iPSC Generation from Human PBMCsSupporting Products and References for iPSC Generation from Human FibroblastsSupporting Products and References for iPSC Generation from Human CD34+ Hematopoietic ProgenitorsReferences for iPSC Generation from Human Bone Marrow Mononuclear Cells Grab a coffee and check out our free webinars. StemRNA - NM Reprogramming kit - next generation of RNA based IPSC reprogramming. CytoTune™-iPS Reprogramming Kit 3 pack (3 vials of each gene) A13780-02 1. This system produces transgene-free, virus-free human induced pluripotent stem cells (iPSCs) with efficiencies in the range of 0.04% to 0.3%, depending upon the cell … Typical reprogramming factors used in different combinations are Oct4, Sox2, Klf4, c-Myc, Nanog and Lin28. Generation of induced pluripotent stem cells (iPSCs) from somatic cells is no longer an art; it’s predictable science with CytoTune-iPS Sendai reprogramming kits. For example, since the introduction of our reprogramming technology in 2012, we have increased the number of sources your RNA-iPSCs can be derived from and have extended our human tissue network to allow rapid access to patient samples (Figure 2).Whether you have your own cells that require reprogramming or wish to source tissues from a select patient group, our expert scientists have the resources and expertise to achieve your research goals. they can indefinitely self-renew in culture and have the potential to differentiate into cell types from all three germ layers i.e. Retrovirus iPSC Reprogramming Kit: #RF101 Product Description Human induced pluripotent stem cells (iPSCs) can be derived from somatic cells through a reprogramming process driven by ectopic expression of a defined set of reprogramming factors: Oct4, Sox2, Klf4 and c-Myc. For human cells, iPSC generation by cellular reprogramming was first described in 2007 by two independent research groups (Yu J et al. ✓ Integration-free No DNA is used during reprogramming, eliminating the risk of genomic integration of reprogramming factors or vector sequences 3. You can access our custom iPSC services FAQs here, or find out more on our blog.If you would like to find out more about the science behind REPROCELL’s unique reprogramming technology, you can access our At REPROCELL, you can gain access to the most rapid integration-free reprogramming method on the market: StemRNA™ 3rd Gen reprogramming technology. ✓ Footprint-Free mRNA vectors are rapidly degraded 48 hours following transfection, meaning there is no need to screen your iPSCs for vector retention 2. Our CytoTune-iPS 2.0 Sendai Reprogramming Kits support cutting-edge basic research, and our CTS CytoTune 2.1 Sendai Reprogramming … Potential inactivation of tumor suppressor genes by random integration and long-term presence of reprogramming factors may give rise to tumor growth. The single RNA contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1, and allows for efficient reprogramming … (2007) Science 318 (5858): 1917–1920; Takahashi K et al. Although typical clinical samples used for REPROCELL reprogramming services are human blood or urine, other options may be possible including alternative species and tissues or cell-lines. As they can be “made” from a person’s own skin or blood, reprogramming can provide a source of patient-specific, genetically identical cells that would not be rejected by the immune system.The raise of iPSCs makes the usage of pluripotent stem cells independent from the availability of embryonic cells and thus independent of ethic controversy and patient-/tissue-matching issues.For human cells, iPSC generation by cellular reprogramming was first described in 2007 by two independent research groups (Yu J et al. Lonza’s NucleofectorArnold et al (2012) ISRN Cell Biol, Article ID 124878Baghbaderani et al. REPROCELL USA Inc.

Expression of these factors in various adult cell types including fibroblasts, peripheral blood mononuclear cells (PBMCs), fibroblasts or CD34+ hematopoietic progenitor was shown to be able reverting those cells back into a pluripotent stem cell state.
DISCLAIMER: TO THE EXTENT … Registered office REPROCELL USA Inc., 9000 Virginia Manor Road, Suite 207, Beltsville, 20705, USA, Registered in United StatesPlease click here to see current operating statuses for all REPROCELL offices. StemRNA™ is the ideal vector for introducing reprogramming factors into cells, because RNA is: 1. The Simplicon™ RNA Reprogramming Technology utilizes a single polycistronic self-replicating RNA strand that is sufficient for generating high numbers of human iPSCs using a single transfection step. Time course of human iPSC colonies generation using Human Simplicon RNA Reprogramming Kit. Lonza’s non-viral NucleofectorDownload a more detailed guideline on transfection of human induced pluripotent stem cells using NucleofectorDownload more detailed guideline on Genome Editing using NucleofectorKeep up to speed on the latest scientific developments, events, tips and tools from Lonza.Need a break from your daily routine? (2007) Cell 131 (5): 861–872). We use cookies to deliver the best service to you. Virus-Free iPS Reprogramming Kits.
However, it remains to be assessed in more detail, whether they can be considered fully identical to natural pluripotent cells.

Thus, researcher have explored alternatives by using non-integrating RNA viruses or non-viral delivery of episomal vectors or even mRNA. 2 ™ CytoTune -iPS 2.0 Sendai Reprogramming Kit User Guide Information in this document is subject to change without notice. The CytoTune™-iPS Reprogramming Kit is used to reprogram somatic cells into induced pluripotent stem cells (iPSCs). When iPSC colonies reach approximately 200 cells or over in size, they are ready to be picked and expanded in feeder-free PluriSTEM Human ES/iPSC Media for further experiments. The expression of these transcription fac-


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